• Stained tissue creates a large and unique gamut of colours.
• Digital Pathology needs to account for this colourimetric biochemistry.
• FFEI’s patented Sierra technology precisely mimics biologically stained tissue.
• This unique feature creates coloured patches with spectral transmittance near-identical to the gamut of stained tissue.
• Different stains at different concentrations produces a metamerism-free range of hisological/cytological colours.
• Spectral data for each patch is obtained using a high-precision spectrophotometer.
• Equipment calibrated to National Physics Laboratory standards and ICC values ensures spectral accuracy.
• This spectrally ‘true’ data is unique to each individual slide.
• Eliminates variation in production and enhances precision and traceability.
• The Sierra slide is scanned by the WSI instrument to be calibrated.
• Output data contains the colour intensities interpreted by the non-calibrated scanner.
• Colour interpreted by the scanner can be compared to original Sierra data, known to be ‘true’ to precise ICC values.
• The accuracy of colour scanned relative to truth represents that WSI systems error across the stained tissue gamut.
• With ‘error’ for each colour quantified, generic colour profiling can be updated with the device-specific ICC profile.
• The scanner’s fidelity to colour ‘truth’ is quantified, calibrated and corrected.
• Crucially, this process does not interfere with the tissue image data itself – all pathology data remains for the pathologist.
• This parallel metadata allows for software to represent the true colour data of each image.